Rory McIlroy will lead Europe's attempt to win a fourth consecutive Ryder Cup at Hazeltine National on Sunday.
Darren Clarke's men will head into the final matches of the contest trailing the United States 9½-6½ and needing to take 7½ points from the 12 singles contests to bring the trophy back across the Atlantic with them.
McIlroy has won three points from four contests so far in Minnesota and will go up against the in-form Patrick Reed at 1104 on Sunday morning.
The American was in inspired form in Saturday's fourballs, winning six holes for the home side alongside Jordan Spieth in a 2 and 1 victory over Henrik Stenson and Justin Rose.
Reed has won 2½ points from his four matches with Spieth so far this week and with him and McIlroy both showing their passion and playing up to the huge galleries, a highly charged encounter is to be expected.
Open champion Stenson will then go out against Spieth, the pair meeting for a fourth time this week after facing each other in the foursomes on Friday and in both fourball sessions, with the American leading 2-1.
Belgian rookie Thomas Pieters will face off against JB Holmes in match three, looking to claim a fourth point from five in what has already been an outstanding debut performance.
Olympic champion Justin Rose will then put his undefeated singles record to the test against Rickie Fowler, before rookie Rafa Cabrera Bello - undefeated in two matches this week - goes head-to-head against US PGA Championship winner Jimmy Walker.
A clash of Ryder Cup heavyweights comes in match six as Sergio Garcia takes on Phil Mickelson in a tussle between two players taking their combined appearance total to 19.
Lee Westwood - making his tenth appearance this week - will then take on American rookie Ryan Moore before English first-timer Andy Sullivan faces Brandt Snedeker.
BMW PGA Championship winner Chris Wood faces World Number Two Dustin Johnson in his first singles match before two rookies go up against each other in the clash between Masters champion Danny Willett and Brooks Koepka.
Martin Kaymer holed the putt to retain the trophy at Medinah in 2012 and he goes out 11th again against Matt Kuchar, with rookie Matthew Fitzpatrick anchoring the singles against Zach Johnson.
"We've gone very strong," said Clarke. "The whole team's strong, but we'll go very strong there early.
"So there's some wonderful games that we're all going to see tomorrow out there, starting off with the first one with Patrick Reed, who played fantastically well today, against Rory who has played fantastically well all week.
"I think the fact that it's come out in the draw, it's a mouth watering prospect for the first match off tomorrow morning."
While that first match may set the tone for the singles session, Clarke insists he has given deep thought to every single one of his 12 choices, including putting Kaymer in his position from Medinah.
"It wasn't randomly, no," he said. "Martin has worked so hard this week and has been a great part of the team. He has spoken up in team meetings, as I would expect from a double Major Champion.
"He's down there tomorrow. We've gone through the list trying to put people where we wanted to put them specifically. Justin Rose, for example, likes playing at number four. He's had great success playing at number four. And Martin, as well, is down there for that reason.
"Matt's game is very strong. He hits fairways, he hits greens, that's what he does. That's what Matt's strengths are. And to have somebody who hits fairways and who hits greens, if it really comes down to it, that's what you want.
"Matt could be in a wonderful position tomorrow afternoon. I certainly hope he is."
1104 - Patrick Reed v Rory McIlroy
1115 - Jordan Spieth v Henrik Stenson
1126 - JB Holmes v Thomas Pieters
1137 - Rickie Fowler v Justin Rose
1148 - Jimmy Walker v Rafa Cabrera Bello
1159 - Phil Mickelson v Sergio Garcia
1210 - Ryan Moore v Lee Westwood
1221 - Brandt Snedeker v Andy Sullivan
1232 - Dustin Johnson v Chris Wood
1243 - Brooks Koepka v Danny Willett
1254 - Matt Kuchar v Martin Kaymer
1305 - Zach Johnson v Matthew Fitzpatrick